FastQCFastQC Report
Mon 6 Mar 2023
EGAF00002800126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002800126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82326090
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGTACAATCTCGTAT25280313.070753147635215TruSeq Adapter, Index 7 (97% over 36bp)
GAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATTTTGG1729870.21012415383750158No Hit
GGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTTG1400020.17005787594187943No Hit
GTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCCTAAGATAGAG1373450.16683046650217448No Hit
GTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATTATTCCTT1337370.16244789470749796No Hit
CGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTT1278420.15528734572478783No Hit
GGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTATTAG1265610.15373133838859587No Hit
GGCGCTTCCAATTAGGTGCATGAGTAGGTGGCCTGCAGTAATGTTAGCGG1234770.14998525983682692No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGTACAATCTCGTATG1185370.14398473193613348TruSeq Adapter, Index 7 (97% over 35bp)
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT1143580.13890857685576954No Hit
GTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGAGGG1099860.13359798819547972No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT1094990.13300643817773927Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTTTGGATGAGAATGGCTGTTACTACGAGGGCTATGTGGCTGATTGAAGA1072460.13026976016958902No Hit
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATG1071530.13015679476579053No Hit
GTAGGTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGA1059930.12874776392271273No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG1046320.12709458204561883No Hit
GTTGAGAATGAGTGTGAGGCGTATTATACCATAGCCGCCTAGTTTTAAGA1024280.12441742344377099No Hit
CCGGAGAGTATATTGTTGAAGAGGATAGCTATTAGAAGGATTATGGATGC984430.11957691662509418No Hit
GGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTATTAGTGGGA979040.11892220315576751No Hit
GTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCC970110.11783749234294985No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACAAGGTACAATCTCGTAT963570.11704309047107667Illumina Single End PCR Primer 1 (96% over 25bp)
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT896920.1089472365321856No Hit
GCGGGTTTTAGGGGCTCTTTGGTGAAGAGTTTTATGGCGTCAGCGAAGGG894660.10867271845413767No Hit
GCGGTCTGTTAGTAGTATAGTGATGCCAGCAGCTAGGACTGGGAGAGATA891800.10832531947041332No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCAC887300.10777871267784005No Hit
CTAGGCTGGAGTGGTAAAAGGCTCAGAAAAATCCTGCGAAGAAAAAAACT874430.10621541725108043No Hit
GGGACGATTAGTTTTAGCATTGGAGTAGGTTTAGGTTATGTACGTAGTCT870360.10572104177424192No Hit
GTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGT852280.10352489714985857No Hit
GTGAGTGTTAGGAAAAGGGCATACAGGACTAGGAAGCAGATAAGGAAAAT850670.10332933338629345No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)