FastQCFastQC Report
Sun 5 Mar 2023
EGAF00002800128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002800128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73568129
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTAGAATCTCGTAT15243402.0720113732945418TruSeq Adapter, Index 20 (97% over 37bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCCGACAACATCTCGTAT4580090.622564425962226Illumina Single End PCR Primer 1 (96% over 25bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACATGCCTAAATCTCGTAT946530.1286603333353768Illumina Single End PCR Primer 1 (96% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)