FastQCFastQC Report
Thu 23 Mar 2023
EGAF00002800130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002800130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56339563
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGATCATCTCGTAT23343084.143283823482976TruSeq Adapter, Index 2 (97% over 38bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACAAGGTACAATCTCGTAT893200.1585386808910818Illumina Single End PCR Primer 1 (96% over 25bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGATCATCTCGTATG571940.10151658435831319TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)