FastQCFastQC Report
Thu 12 May 2022
EGAF00002800132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002800132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177767313
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT18601051.0463706564547104Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTCACCATCTCGTAT13765120.7743335806622672TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTCACCATCGCGTAT2274130.12792734286308305TruSeq Adapter, Index 1 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTCACCATCTCGTA2194140.12342764049091523TruSeq Adapter, Index 1 (97% over 36bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG1817410.10223533051883392Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)