FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002838539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002838539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14857725
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA597020.40182464004415214Illumina RNA PCR Primer (100% over 45bp)
CGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT590890.3976988401656377Illumina RNA PCR Primer (100% over 44bp)
GCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG279250.1879493664070374Illumina RNA PCR Primer (100% over 43bp)
GGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT245180.16501853412955214Illumina RNA PCR Primer (100% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG205980.13863495252469674No Hit
CTGCCTGATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGC196300.1321198231896202No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)