FastQCFastQC Report
Sun 2 Apr 2023
EGAF00002838540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002838540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36735864
Sequences flagged as poor quality0
Sequence length35-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1714800.46679179779193436No Hit
CGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT1499000.40804811341853836Illumina RNA PCR Primer (100% over 44bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1497440.4076234602784897Illumina RNA PCR Primer (100% over 45bp)
GCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG726030.19763520465994758Illumina RNA PCR Primer (100% over 43bp)
GGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT587630.15996084915819594Illumina RNA PCR Primer (100% over 44bp)
CTGCCTGATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGC510510.13896774008091928No Hit
GACGGCTTACAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCG394260.1073229147407558No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)