FastQCFastQC Report
Wed 14 Sep 2022
EGAF00002838542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002838542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34430686
Sequences flagged as poor quality0
Sequence length60-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA2872780.8343661813766939No Hit
TCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCT1952720.567145249444057No Hit
TCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTG1734510.5037686440519948No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1560080.4531074402641876No Hit
CTGCCTGATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGC1381610.4012728645604099No Hit
GCTCCAATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTTTGCTGGATA1073210.3117016024600846No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG993740.28862044746944626No Hit
CCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTTGAGGACGACGCT981220.28498415628430984No Hit
CGCCTTATATTTAAAGACAAAAAGAAGTGTCTAACAACAACATCCACACT948820.2755739458691006No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT900740.261609658314679No Hit
TGCCTGATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCG840240.24403812343442707No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA718920.20880211332414347No Hit
GCCTGATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGA697650.2026244844497144No Hit
ACTGCCTGATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTG674040.19576722926752027No Hit
GTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAGCGATTCAGCGC655090.19026341792899507No Hit
GGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGT586920.17046421903995757No Hit
CCCTGCATCCATTATTTTCTAAAACTTTAACTGCTTCCCAAATTTCCCCA578520.16802453485823662No Hit
GACGGCTTACAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCG571820.16607859628472113No Hit
TCCCTGCATCCATTATTTTCTAAAACTTTAACTGCTTCCCAAATTTCCCCAATATCAGACATTCCTGTAGATAAA571100.1658694804977165No Hit
CCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGA562040.16323810684457463No Hit
ACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGG554880.1611585665182506No Hit
ACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAAT552490.16046441828083238No Hit
GCATCCATTATTTTCTAAAACTTTAACTGCTTCCCAAATTTCCCCAATAT481800.13993331413727858No Hit
TGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAGCGATT455080.13217279493066156No Hit
TCACCGGCTTGCCTGTTTTTGCCACTTTTTCTAATAAGGGATAAAAGGTTAAATCACCAGAGGCAATTTTAAATC445660.1294368633840174No Hit
ATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGTT434190.12610553272159608No Hit
TCTCCTGGAAAAACCTTTGCTGGATATTTGTTAAATATCAATAGCCCTAA427240.12408698449981508No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCTA422340.1226638353938112No Hit
TCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAA421140.12231530908213678No Hit
CCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGTATA401490.1166081907284682No Hit
TCCCCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGT401060.11648330213345154No Hit
GGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAGCGATTCAGCGCTATTTGCGCCAAGAACTGTT397100.11533316530492597No Hit
TTATTTTCTAAAACTTTAACTGCTTCCCAAATTTCCCCAATATCAGACAT386450.1122399942888155No Hit
TCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCCTGATTTT374130.10866179082229148No Hit
GATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCTGAGGGA370750.10768010837774189No Hit
ACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCAT355730.10331772070995042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)