FastQCFastQC Report
Mon 18 Sep 2023
EGAF00002839194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002839194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28131267
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGG2739640.9738772164083473No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA1763170.6267652288821545No Hit
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG1141070.4056233940689553No Hit
TTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC918520.3265121332786042No Hit
CCACCTACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG878390.3122468675157788No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC790510.2810076062340171No Hit
AAACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC755500.2685623793624368No Hit
AGGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC740760.2633226580231882No Hit
CAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGC701230.24927067806793063No Hit
TGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCA607780.21605141353924798No Hit
GGGTTGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTA556800.19792922942290514No Hit
ACGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACC492020.1749014717325032No Hit
CCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG431750.1534769123623191No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGT398300.1415862285904151No Hit
GGTGAAGTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTC387520.13775419358111385No Hit
GTGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAAT387240.1376546602042489No Hit
AGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTA363970.12938272563407827No Hit
CAGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA363280.12913744695537532No Hit
CGTAGCGACGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGC338790.12043183124315018No Hit
ACCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACT333520.11855847090001313No Hit
ATCCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAG304140.10811457585611058No Hit
GTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGA303090.10774132569286694No Hit
TTCGGTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTC284840.10125388237934681No Hit
ACGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAAT282970.1005891416124272No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)