FastQCFastQC Report
Mon 18 Sep 2023
EGAF00002839196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002839196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28083989
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGG2702930.9624451854043955No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA1735520.6179748895358135No Hit
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG1126510.40112179220694044No Hit
TTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC905280.3223473702400325No Hit
CCACCTACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG870250.30987407095195774No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC776770.27658820119891087No Hit
AAACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC739490.2633137336722358No Hit
AGGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC735560.26191435981548067No Hit
CAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGC694890.24743279880931443No Hit
TGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCA594920.21183600378137166No Hit
GGGTTGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTA547810.19506132123894507No Hit
ACGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACC483460.17214790961497672No Hit
CCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG431920.15379581582943933No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGT396340.14112667541637336No Hit
GGTGAAGTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTC387510.13798253517333312No Hit
GTGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAAT380190.13537606783708683No Hit
AGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTA357940.1274534041442617No Hit
CAGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA351850.12528490877845025No Hit
CGTAGCGACGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGC335810.11957346942416192No Hit
ACCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACT328200.11686374040382938No Hit
ATCCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAG302660.10776959070878428No Hit
GTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGA299410.10661234769747274No Hit
TTCGGTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTC282220.1004914223545665No Hit
ACGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAAT282120.10045581487729538No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)