FastQCFastQC Report
Wed 22 Feb 2023
EGAF00002839304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002839304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331262014
Sequences flagged as poor quality0
Sequence length26
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACA31801110.9599986915493426No Hit
GTCTCGTTCGTTATCGGAATTAACCA31622100.9545948120692159No Hit
AGGCCGTATCGTTCCGCCTGGGCGGG26627490.8038196012416926No Hit
CCACCTACTAAGAACGGCCATGCACC26106020.7880776816142885No Hit
TTGCCGTATCGTTCCGCCTGGGCGGG24040210.7257158679232083No Hit
AAGCCGCAGGCTCCACTCCTGGTGGT14906300.44998518906547497No Hit
TGTTCCGCCTGGGCGGGATTCTGACT11409610.34442856463463994No Hit
ACGCCAGAGTCTCGTTCGTTATCGGA11222930.3387931463823075No Hit
CCACCAACTAAGAACGGCCATGCACC10552330.3185493522960951No Hit
GGGTTGCGTAACTAGTTAGCATGCCA10184950.3074590375460315No Hit
ACACCAAATGTCTGAACCTGCGGTTC9418600.2843247822552935No Hit
ACGGGCTTTTTAACTGCAGCAACTTT9298940.2807125359082071No Hit
ACGGCCATGCACCACCACCCACGGAA9268050.2797800414266635No Hit
CTCGTACTGAGCAGGATTACCATGGC9202680.277806679035647No Hit
CGTCCATTCTCGACTGCCGGCGACGG9109360.2749895736611684No Hit
ACACCCTCATGTCTCTTCACCGTGCC8524010.25731927114347614No Hit
GACCTGGGCGGGATTCTGACTTAGAG7995170.2413548690191807No Hit
GTGGGTCGCGTAACTAGTTAGCATGC7854690.2371141171652721No Hit
GGGTCTGCGAGAGCGCCAGCTATCCT7748220.23390004505617717No Hit
GCTTCCAATGATAGGAAGAGCCGACA7604820.2295711454558747No Hit
TGCCCTCCAATGGATCCTCGTTAAAG7159990.21614280229546634No Hit
CTACACCTCTCATGTCTCTTCACCGT6324340.19091654740709268No Hit
TGCCCTACAATGGATCCTCGTTAAAG6269000.18924596648742226No Hit
GATCGCGTAACTAGTTAGCATGCCAG6012970.181517039258235No Hit
ATCCACCAACTAAGAACGGCCATGCA5273020.15917973619516784No Hit
GCTTCACAATGATAGGAAGAGCCGAC5223320.15767941325140888No Hit
GAACCTACGGTTCCTCTCGTACTGAG5185400.15653470005166364No Hit
CGGACGTTATCGGAATTAACCAGACA5027110.15175630731992107No Hit
ACCTTTAAGTTTCAGCTTTGCAACCA4665850.140850740586272No Hit
CGCTTCACCAACTAAGAACGGCCATG4507050.13605695218649488No Hit
GGCCGTATCGTTCCGCCTGGGCGGGA4408150.13307140009116772No Hit
CTTACCGTGCCAGACTAGAGTCAAGC4220340.12740186986848423No Hit
TGGCCAGAGTCTCGTTCGTTATCGGA4211040.12712112533373657No Hit
GCTCCACCAACTAAGAACGGCCATGC4112300.12414040325191043No Hit
GACGCGTAACTAGTTAGCATGCCAGA3945390.11910179354279962No Hit
ACGGGTCGCGTAACTAGTTAGCATGC3801430.11475598889524351No Hit
ACGGGTCTGCGAGAGCGCCAGCTATC3767520.11373232790886792No Hit
CCTACCAACTAAGAACGGCCATGCAC3703910.11181209566636277No Hit
GTCGGGTCTGCGAGAGCGCCAGCTAT3425230.1033994196509353No Hit
TTGGCAACAACACATCATCAGTAGGG3393210.10243281319904068No Hit
ACACCTCTCATGTCTCTTCACCGTGC3368050.10167329357600297No Hit
GCAGCCATACTCCCCCCGGAACCCAA3353160.10122380044456289No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)