FastQCFastQC Report
Fri 30 Dec 2022
EGAF00002839305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002839305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331262014
Sequences flagged as poor quality0
Sequence length98
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTC6904770.20843832700962808No Hit
CTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTT6173560.18636486343405495No Hit
CCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTT6008580.18138451576280037No Hit
GTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAG5718770.17263585193320716No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT5288660.159651870014894No Hit
GAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTTC5264270.15891559483183001No Hit
GCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGC4590560.13857791735819128No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4492330.13561259094439967Clontech SMART CDS Primer II A (100% over 26bp)
CTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAG4271360.12894204042362672No Hit
ATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATC4269580.1288883065234277No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGT4232570.1277710640254696No Hit
GTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGA4143040.12506836959579676No Hit
GAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCA4042510.1220336117379278No Hit
GGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAG3971450.11988848199178068No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGA3949970.11924005267926675No Hit
CAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTA3903250.11782968873696457No Hit
GGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGA3789110.11438407785566383No Hit
GCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTTCTGTCCTCCCTGAGCT3730420.11261236852831548No Hit
GGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTG3660360.11049742636654984No Hit
GTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAA3636470.10977624497567656No Hit
CAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAAT3577340.10799125311120036No Hit
CTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATT3545400.10702706166605627No Hit
GATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTTCTGTCCTCCC3516920.10616731926287208No Hit
AAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCT3507670.1058880841073435No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC3468330.10470050453777655No Hit
CAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAA3465080.10460239488853677No Hit
GTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGT3421630.10329074434716201No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)