FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002839331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002839331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38221671
Sequences flagged as poor quality0
Sequence length31
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAATTCAAAAAAAAAAAAAAAAAAAAAN1195590.3128042204120275No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAN1005590.26309420119282595No Hit
CCTCTGCAAAAAAAAAAAAAAAAAAAAAAAN909550.23796709463592003No Hit
ATATGGGTGTAAAAAAAAAAAAAAAAAAAAN720350.18846638076079927No Hit
GTAGTCACAGCGAAAAAAAAAAAAAAAAAAN707100.18499976099946022No Hit
CCTCTGCAATAAAAAAAAAAAAAAAAAAAAN695820.18204855564792025No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAN620120.16224303746426993No Hit
CATCATCTTACAAAAAAAAAAAAAAAAAAAN619000.16195000998255676No Hit
CTGAGGCCCAAAAAAAAAAAAAAAAAAAAAN616910.16140319977114553No Hit
CGGCCAAAAAAAAAAAAAAAAAAAAAAAAAN616900.16140058345434452No Hit
TACAAAAAAAAAAAAAAAAAAAAAAAAAAAN601120.15727203554234978No Hit
TTGCTCGAAAAAAAAAAAAAAAAAAAAAAAN570370.14922686137924215No Hit
TACGATGGGACAAAAAAAAAAAAAAAAAAAN567030.1483530115677046No Hit
GTGCCACTCAAAAAAAAAAAAAAAAAAAAAN563610.14745823122175897No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAN537570.14064534227192738No Hit
TGGTCCAAAAAAAAAAAAAAAAAAAAAAAAN521410.1364173743214942No Hit
TACGATGGGACAATCAAAAAAAAAAAAAAAN508410.13301616248018042No Hit
TTCTCAGATGTAAAAAAAAAAAAAAAAAAAN505570.1322731285086934No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAN494250.1293114578899494No Hit
GACTCAAAAAAAAAAAAAAAAAAAAAAAAAN488350.12776783097735314No Hit
GGCTCAAAAAAAAAAAAAAAAAAAAAAAAAN474270.12408405692153021No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAN473540.12389306579505643No Hit
ATATTGCCACCCTACCAAAAAAAAAAAAAAN452430.11837002102812304No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAN434210.1136030918166817No Hit
GTACCAAAAAAAAAAAAAAAAAAAAAAAAAN431420.11287313942919973No Hit
TCAACTCACAAAAAAAAAAAAAAAAAAAAAN414930.10855883302433324No Hit
CATTTACGAGACCCGAAAAAAAAAAAAAAAN406920.10646316326672373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)