FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002839365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002839365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35432474
Sequences flagged as poor quality0
Sequence length31
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGCAAAAAAAAAAAAAAAAAAAAAAAN1320750.37275127895387716No Hit
TCCGAATTCAAAAAAAAAAAAAAAAAAAAAN1112980.314112980087137No Hit
CCTCTGCAATAAAAAAAAAAAAAAAAAAAAN1035350.2922036999166358No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAN907150.2560222015544272No Hit
CATCATCTTACAAAAAAAAAAAAAAAAAAAN740430.20896932006497768No Hit
GTAGTCACAGCGAAAAAAAAAAAAAAAAAAN693030.19559176138815482No Hit
GTGCCACTCAAAAAAAAAAAAAAAAAAAAAN658130.1857420399151355No Hit
CTGAGGCCCAAAAAAAAAAAAAAAAAAAAAN652680.18420390289427716No Hit
TTCTCAGATGTAAAAAAAAAAAAAAAAAAAN625170.1764398387761464No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAN604300.1705497617806761No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAN585350.16520156057971003No Hit
ATATGGGTGTAAAAAAAAAAAAAAAAAAAAN565650.15964168914651572No Hit
TACAAAAAAAAAAAAAAAAAAAAAAAAAAAN540310.15249005756696526No Hit
TACGATGGGACAAAAAAAAAAAAAAAAAAAN535020.15099707686231562No Hit
TGGTCCAAAAAAAAAAAAAAAAAAAAAAAAN534160.15075436166269396No Hit
GACTCAAAAAAAAAAAAAAAAAAAAAAAAAN530310.14966778780392248No Hit
TACGATGGGACAATCAAAAAAAAAAAAAAAN495780.1399224903121357No Hit
TTGCTCGAAAAAAAAAAAAAAAAAAAAAAAN490230.13835613059364696No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAN460990.13010381380650982No Hit
GTACCAAAAAAAAAAAAAAAAAAAAAAAAAN440600.12434920575966557No Hit
ATATTGCCACCCTACCAAAAAAAAAAAAAAN435430.12289009229217243No Hit
CATCATCTTAAAAAAAAAAAAAAAAAAAAAN424800.11989001953405794No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAN419860.11849581827111479No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAN416180.11745722299831504No Hit
TCAACTCACAAAAAAAAAAAAAAAAAAAAAN411910.11625211380949577No Hit
CGGCCAAAAAAAAAAAAAAAAAAAAAAAAAN410200.11576950568001547No Hit
ACCTTAGCTGGGCTACAAAAAAAAAAAAAAN384780.10859529594236068No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAN383700.10829049080795204No Hit
TTGCTCGAATAAAAAAAAAAAAAAAAAAAAN383200.10814937731979991No Hit
GGCTCAAAAAAAAAAAAAAAAAAAAAAAAAN365550.10316807118802937No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)