FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002854452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002854452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390802484
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG7639070.19547137781242965TruSeq Adapter, Index 8 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1812450.083.790931
TCGGAAG1951050.077.728072
CGGAAGA2143900.070.634473
AGAGCAC4477650.034.2258457
AGCACAC4807900.031.9259059
GAAGAGC4891250.031.4461545
GAGCACA4893150.031.4202548
CGCGCAT1317000.022.44806135-39
AAGAGCA7040450.022.1216096
TCGCGCA1364450.021.90009535-39
TCTCGCG1393300.021.72524630-34
TATGCCG1460500.020.8071745-49
GCGCATC1473750.020.20702635-39
GGAAGAG7924600.019.7471834
CTCGCGC1557800.019.36875730-34
CGTATGC1574300.019.16127645-49
CTCGTAT1308650.018.75924940-44
TCTCGTA1314400.018.45878840-44
ATGCCGT1711000.017.66428445-49
ACTCTCG1740000.017.55473730-34
Produced by FastQC (version 0.11.5)