FastQCFastQC Report
Fri 22 Apr 2022
EGAF00002854646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002854646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370887990
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG3979280.10729061353536952TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1239850.062.7402651
TCGGAAG1340950.057.8897632
CGGAAGA1509100.051.4967963
AGAGCAC3767600.021.1296887
AGCACAC4074800.019.709869
GAGCACA4158750.019.2041078
GAAGAGC4199050.019.0952175
TATGCCG918150.017.0715245-49
CGTATGC1016950.015.3331345-49
CTCGTAT882600.014.64724240-44
TTACTCG1086050.014.42084530-34
TCTCGTA903750.014.31889740-44
TACTCGA1128300.013.89191335-39
ATGCCGT1140450.013.8354245-49
TGCCGTC1127500.013.816874545-49
GCCGTCT1153900.013.46830350-54
AAGAGCA6348400.012.9327576
TCGTATG1001700.012.87821840-44
CGTCTGA1307400.012.199800515-19
ACACGTC1317250.012.14149110-14
Produced by FastQC (version 0.11.5)