FastQCFastQC Report
Fri 22 Apr 2022
EGAF00002854719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002854719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406889128
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC10359770.25460916222858626Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2101400.078.859559
GAGCGTC2386750.069.44928
ATCGGAA2683850.063.2751351
AGAGCGT2655900.062.5920757
TCGGAAG2816600.059.8735242
CGGAAGA2965600.056.621173
AAGAGCG3048950.054.9535066
GAAGAGC5861300.029.1381915
CGCCGTA1509650.021.63880355-59
TCGCCGT1596100.020.4540655-59
GTCGCCG1739050.019.3645455-59
GCCGTAT1722300.019.08828555-59
GGAAGAG9157300.019.0064774
TGGTCGC1863450.018.1849150-54
CCGTATC1828300.017.616155-59
GGTCGCC1918500.017.44388450-54
CGTATCA1893850.017.22270660-64
GTGGTCG1984300.017.1848350-54
GCGTCGT2030100.016.52279910-14
CGTCGTG2060700.016.31543210-14
Produced by FastQC (version 0.11.5)