FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002854777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002854777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369869034
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1185450.063.512051
TCGGAAG1299550.057.7703972
CGGAAGA1448850.051.762073
AGAGCAC3743150.020.5842367
AGCACAC4083050.018.9690389
GAGCACA4164750.018.5448578
GAAGAGC4204250.018.4992585
TATGCCG897050.016.94126745-49
CGTATGC985450.015.37741245-49
CTCGTAT842000.014.09491440-44
ATGCCGT1097900.013.89617245-49
TCTCGTA865400.013.64008340-44
TGCCGTC1105250.013.58600945-49
GCCGTCT1126650.013.23939850-54
TCGTATG919000.012.75934840-44
AAGAGCA6399500.012.5158016
CGTCTGA1277200.012.16603415-19
ACACGTC1281300.012.14510810-14
ATCTCGT1045000.011.49559540-44
GGAAGAG7131200.011.2745444
Produced by FastQC (version 0.11.5)