FastQCFastQC Report
Fri 22 Apr 2022
EGAF00002854826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002854826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351312371
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC15896690.45249445542582384Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2923400.087.591019
GAGCGTC3184650.080.409638
AGAGCGT3409400.075.267137
ATCGGAA3487650.074.943561
TCGGAAG3593800.072.387622
CGGAAGA3734600.069.516143
AAGAGCG3782950.068.312026
GAAGAGC6276550.041.534395
GGAAGAG9175800.028.88264
CGCCGTA2152550.023.70269455-59
TCGCCGT2205200.023.06907855-59
GTCGCCG2367550.022.04431555-59
GCCGTAT2322550.022.03142555-59
TGGTCGC2475200.021.28393650-54
CCGTATC2423700.020.54200755-59
CGTATCA2473100.020.44433860-64
GTGGTCG2600200.020.34608550-54
GGTCGCC2554600.020.16540350-54
TCTCGGT2861100.018.64301345-49
GCGTCGT2817500.018.46304710-14
Produced by FastQC (version 0.11.5)