FastQCFastQC Report
Fri 22 Apr 2022
EGAF00002854857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002854857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431006380
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG15807200.3667509515752412TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA4116400.069.929871
TCGGAAG4151450.069.343712
CGGAAGA4298250.066.865183
AGAGCAC6692050.043.1778037
AGCACAC6901000.041.9171879
GAGCACA7092150.040.7475748
GAAGAGC7302850.039.684375
AAGAGCA9727900.030.0091696
GGAAGAG10836800.027.1041184
TATGCCG2481550.023.37324145-49
CTCGTAT2524400.022.30070340-44
CGTATGC2591050.022.14027845-49
TCTCGTA2292100.021.5170740-44
ATGCCGT2669250.021.22538245-49
TCGTATG2721800.021.14215540-44
TGCCGTC2648500.021.11620745-49
TACTCGA2711650.021.06065235-39
GCCGTCT2645000.020.9057950-54
TTACTCG2749150.020.90413930-34
ACTCGAT2513450.020.0455535-39
Produced by FastQC (version 0.11.5)