FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002854907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002854907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433439813
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1473000.021.5489371
TCGGAAG1566150.020.2992482
CGGAAGA1730150.018.5213933
TATGCCG647550.010.35806245-49
CGTATGC749600.08.99436645-49
CTCGTAT759050.08.84713140-44
AGAGCAC4341400.07.9088657
TCTCGTA728550.07.728371640-44
TCGTATG873850.07.63340540-44
ATGCCGT885700.07.590966745-49
AGCACAC4628600.07.51050239
GCCGTCT887900.07.373057450-54
TGCCGTC878900.07.34932245-49
GAGCACA4813450.07.231118
GAAGAGC4912950.07.0860355
ATCTCGT956200.06.1280840-44
CCGTCTT1173000.05.88276550-54
CGTCTTC1179600.05.826484750-54
ACACGTC1332500.05.30476310-14
CGTCTGA1347700.05.22242815-19
Produced by FastQC (version 0.11.5)