FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002854925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002854925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339546046
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG3772830.1111139429967033TruSeq Adapter, Index 3 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1782600.045.8598251
TCGGAAG1853050.044.057392
CGGAAGA1966500.041.478373
AGAGCAC3955200.021.0849937
AGCACAC4202800.019.8716499
GAGCACA4292900.019.4637588
GAAGAGC4426500.018.9891515
TATGCCG979400.017.83321845-49
GCGCATC1003400.017.00022335-39
CGTATGC1060400.016.32743545-49
TAATGCG1104450.015.84965130-34
TGCGCAT1140500.015.04561535-39
ATGCCGT1159950.015.01492145-49
CTCGTAT889250.014.93292240-44
ATGCGCA1185000.014.66905235-39
AATGCGC1120850.014.61907630-34
GCCGTCT1177400.014.46447850-54
TCTCGTA910600.014.4187940-44
TGCCGTC1157250.014.06892445-49
AAGAGCA6360500.013.4887066
Produced by FastQC (version 0.11.5)