FastQCFastQC Report
Fri 22 Apr 2022
EGAF00002854948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002854948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363216678
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG5433260.1495872940063617TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2390000.047.195621
TCGGAAG2417400.046.578082
CGGAAGA2495600.045.1006933
AGAGCAC4572950.025.0040637
AGCACAC4827950.023.7669569
GAGCACA4918200.023.305168
GAAGAGC5066250.022.7212585
TATGCCG1196650.019.70516445-49
CGTATGC1289550.018.13492645-49
TCGTATC1353750.017.37566635-39
ATGCCGT1394650.016.86913345-49
GCCGTCT1372450.016.72426450-54
CTCGTAT1095550.016.60512740-44
TGCCGTC1364450.016.43383245-49
AAGAGCA7205250.016.2204576
TCTCGTA1122200.016.1681540-44
GTCACGA1521150.015.53319925-29
ATTCGTA1551350.015.12415535-39
TATCTCG1198600.015.026278540-44
GGAAGAG7874800.014.9216594
Produced by FastQC (version 0.11.5)