FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002854982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002854982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452367157
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG10938650.24180911082366663TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3043000.066.56861
TCGGAAG3153400.064.367222
CGGAAGA3354800.060.433933
AGAGCAC6031600.033.9824527
AGCACAC6276400.032.631639
GAGCACA6515750.031.4996688
GAAGAGC6617550.031.073135
AAGAGCA9195050.022.6458366
TATGCCG1929400.021.33107245-49
GGAAGAG10388950.020.137544
CTCGTAT2022850.020.00635540-44
CGTATGC2048800.019.87062345-49
TCTCGTA1856850.019.07551640-44
TACTCGA2155600.018.8755535-39
TTACTCG2174900.018.75316630-34
ATGCCGT2149050.018.75270545-49
TCGTATG2191150.018.65036640-44
TGCCGTC2141800.018.53923645-49
GCCGTCT2152600.018.26146950-54
ACTCGAT2076850.017.33874335-39
Produced by FastQC (version 0.11.5)