FastQCFastQC Report
Fri 22 Apr 2022
EGAF00002854983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002854983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435079093
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC5462890.1255608483122401Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2039750.042.5500569
GAGCGTC2405950.036.185178
AGAGCGT2783200.031.3225657
ATCGGAA2952550.030.184311
TCGGAAG3027750.029.3656942
CGGAAGA3261350.027.3241063
AAGAGCG3323800.026.727666
CGCCGTA923900.017.36203255-59
TCGCCGT979200.015.995007555-59
GTCGCCG1130600.015.28829955-59
GAAGAGC6337150.014.5218685
GCCGTAT1159400.013.94297755-59
TGGTCGC1307300.013.19196850-54
GGTCGCC1320850.013.00613650-54
CCGTATC1257250.012.58677255-59
GTGGTCG1447950.012.0497450-54
CGTATCA1360400.011.92424460-64
TCCGATC354250.011.6445633
TCTCGGT1777000.010.32198545-49
TCGGTGG1851150.09.66725645-49
Produced by FastQC (version 0.11.5)