FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002854998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002854998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316765472
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG14535050.4588584073961192TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCCCGTATG4353020.13742091183473432TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2969250.0103.545741
TCGGAAG3049700.0100.6379552
CGGAAGA3164550.096.857083
AGAGCAC5089900.060.4192247
AGCACAC5354200.057.4504629
GAGCACA5427500.056.6431128
GAAGAGC5524700.055.7693145
AAGAGCA7386550.041.9171076
GGAAGAG7917950.039.195054
TATGCCG2510350.025.12606245-49
CGGCTAT2574400.024.44752730-34
CGTATGC2574550.024.20665445-49
ATGCCGT2648950.023.59628745-49
CTCGTAT2033450.023.47207840-44
ACCGGCT2696450.023.3818230-34
TCTCGTA2050350.023.16616640-44
GCCGTCT2636700.023.12347250-54
TGCCGTC2642050.022.8627145-49
GTCACCG2778250.022.80285625-29
TCACCGG2733650.022.4042525-29
Produced by FastQC (version 0.11.5)