FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002855020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002855020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402013370
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG4965590.12351803125353766TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1585850.062.9657021
TCGGAAG1709700.058.3183632
CGGAAGA1850550.053.8216253
AGAGCAC4342250.023.427927
AGCACAC4682050.021.7553379
GAGCACA4806500.021.2933868
GAAGAGC4880050.021.0086045
TATGCCG1085300.018.7626545-49
CGTATGC1176100.017.15754545-49
CTCGTAT1011850.016.12197340-44
CGCTCAT1303550.015.56572330-34
TCTCGTA1049500.015.52703140-44
ATGCCGT1311450.015.5072745-49
ACCGCTC1318450.015.38101730-34
TGCCGTC1314200.015.10526945-49
GCCGTCT1326700.014.97271250-54
TCGTATG1079450.014.73090740-44
TATCTCG1101650.014.61434940-44
GTCACCG1413700.014.589437525-29
AAGAGCA7169650.014.58266456
Produced by FastQC (version 0.11.5)