FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002855087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002855087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381682616
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC3880960.10168029240294243Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1272650.050.871939
GAGCGTC1558350.041.6520588
ATCGGAA1781900.037.8457681
AGAGCGT1790550.036.3764657
TCGGAAG1899550.035.0464322
CGGAAGA2050550.032.156683
AAGAGCG2166800.030.5082116
CGCCGTA819600.016.04478655-59
TCGCCGT893050.014.73165555-59
GAAGAGC4849850.014.0979535
GTCGCCG1019550.013.306190555-59
GCCGTAT1011150.013.18736155-59
TGGTCGC1132200.012.13159350-54
CCGTATC1120550.011.50661555-59
GTGGTCG1239600.011.21270450-54
CGTATCA1179250.011.19673860-64
GGTCGCC1210850.011.16393550-54
GCGTCGT1192000.011.16187510-14
CGTCGTG1227250.010.89914910-14
GTCGTGT1434250.09.39180610-14
Produced by FastQC (version 0.11.5)