FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002855155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002855155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432147182
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC14939920.34571369714496947Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3155000.081.9459159
GAGCGTC3526100.073.410338
ATCGGAA4016400.067.677121
AGAGCGT3877950.067.099157
TCGGAAG4097650.066.321912
CGGAAGA4367550.062.2300723
AAGAGCG4454400.060.630146
GAAGAGC7387850.036.9542925
AGCGGCG705900.027.2938489
GGAAGAG11353850.024.4829644
CGCCGTA2061950.023.4885555-59
TCGCCGT2053200.022.77480955-59
GTCGCCG2320150.022.12512255-59
GCCGTAT2262450.021.39480855-59
TGGTCGC2444500.020.5387250-54
CGTATCA2459500.019.5878960-64
GAGCGGC994500.019.5118088
GGTCGCC2584400.019.3332650-54
CCGTATC2412850.019.18247855-59
GTGGTCG2669550.019.16174750-54
Produced by FastQC (version 0.11.5)