FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002855191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002855191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338133037
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC3758230.11114648936241033Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1535500.039.3083429
GAGCGTC1804050.033.5089848
ATCGGAA1970850.031.1214891
AGAGCGT2072900.029.2084377
TCGGAAG2096550.029.1768782
CGGAAGA2295950.026.6454773
AAGAGCG2490100.024.6144546
GAAGAGC4598100.013.7856035
CGCCGTA1002450.011.76274955-59
TCGCCGT1097950.010.76602655-59
GCCGTAT1161000.010.28747955-59
GTCGCCG1280100.09.69720855-59
CCGTATC1240200.09.58376355-59
TGGTCGC1385650.09.13008450-54
CGTATCA1309650.09.10323760-64
GGAAGAG7502000.08.8010524
GGTCGCC1460450.08.60887250-54
GCGTCGT1423400.08.50319910-14
GTGGTCG1521850.08.35678850-54
CGTCGTG1474300.08.25387110-14
Produced by FastQC (version 0.11.5)