FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002855278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002855278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410193432
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC21766360.5306364827411474Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3897900.087.8802269
GAGCGTC4234100.080.949728
AGAGCGT4515750.076.009277
ATCGGAA4628250.075.324071
TCGGAAG4786300.072.570932
CGGAAGA4916300.070.58413
AAGAGCG4995950.069.211356
GAAGAGC7768700.044.882495
GGAAGAG11217100.031.504564
CGCCGTA2916700.023.37497955-59
TCGCCGT3009550.022.6099955-59
GCCGTAT3105200.021.99568755-59
GTCGCCG3230750.021.5101755-59
TGGTCGC3329950.020.97155850-54
CCGTATC3216500.020.81185755-59
CGTATCA3254400.020.80401860-64
GTGGTCG3482100.020.13267350-54
GGTCGCC3430350.020.04110150-54
TCTCGGT3816550.018.51106545-49
GCGTCGT3772500.018.38150210-14
Produced by FastQC (version 0.11.5)