FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002855285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002855285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361574948
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG5619980.1554305692660986TruSeq Adapter, Index 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1416900.077.4612661
TCGGAAG1500200.073.055252
CGGAAGA1646500.066.621373
AGAGCAC3890500.028.6049467
AGCACAC4233100.026.4020589
GAGCACA4292150.026.0844578
GAAGAGC4342100.025.7923665
TATGCCG1134450.019.43949145-49
CGTATGC1210400.018.09991845-49
AAGAGCA6455700.017.5745746
CGATAGA1258200.017.3013735-39
AGCGATA1275600.017.262130-34
GCGATAG1273550.017.15555630-34
CTCGTAT1052250.017.06606140-44
TCTCGTA1061050.016.88899440-44
TGCCGTC1306250.016.71850245-49
ATGCCGT1325000.016.61542145-49
GCCGTCT1322200.016.35922450-54
GGAAGAG7095650.016.136974
TCGTATG1115000.015.96778640-44
Produced by FastQC (version 0.11.5)