FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002855290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002855290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361574948
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC6958500.19244972690973047Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1601450.067.94289
GAGCGTC1886200.057.8218468
ATCGGAA2038900.053.6650241
TCGGAAG2147050.051.013892
AGAGCGT2170450.050.272767
CGGAAGA2354550.046.4052543
AAGAGCG2593450.042.1098676
GAAGAGC4930800.022.6054255
CGCCGTA1283750.016.7873155-59
TCGCCGT1344900.016.01216555-59
GGAAGAG7510200.015.1712894
GCCGTAT1461600.014.90926755-59
GTCGCCG1497100.014.77944355-59
GCGTCGT1527900.014.3292410-14
CGTCGTG1570300.013.98018710-14
TGGTCGC1609550.013.90872150-54
CCGTATC1566700.013.81655655-59
GGTCGCC1664350.013.40459750-54
CGTATCA1645600.013.21575560-64
GTGGTCG1712550.013.15007850-54
Produced by FastQC (version 0.11.5)