FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002855354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002855354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381133704
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC16327670.4283974318891514Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3143150.082.453429
GAGCGTC3477050.074.589198
ATCGGAA3818650.068.399911
AGAGCGT3803550.068.232897
TCGGAAG3924100.066.480162
CGGAAGA4137950.062.9131473
AAGAGCG4293000.060.5443536
GAAGAGC6836750.038.4036835
GGAAGAG9665400.027.5479454
CGCCGTA2206200.023.49971255-59
TCGCCGT2297850.022.51883555-59
GCCGTAT2406100.021.61732555-59
GTCGCCG2489100.021.23238855-59
TGGTCGC2600550.020.47708350-54
CCGTATC2507450.020.14787755-59
CGTATCA2569350.020.1208660-64
GTGGTCG2681350.019.98386650-54
GGTCGCC2663350.019.52547850-54
TCTCGGT3004600.017.9971845-49
GCGTCGT3008100.017.5600210-14
Produced by FastQC (version 0.11.5)