FastQCFastQC Report
Fri 22 Apr 2022
EGAF00002855422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002855422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423757393
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC343750.019.6461223
CCGATCT461850.015.0320234
TTCCGAT528900.012.98842
CTTCCGA640950.011.0224411
CGATCTA308750.07.51101735
CGTCGTG1807550.06.581679145
GCGTCGT1850000.06.493356145
AGCGTCG1961500.06.3423095145
GTACGTC835350.06.291989145
GTCGTGT2034450.05.8903933145
TACGTCC919000.05.545724145
TCGTGTA2012900.05.413214145
GAGCGTC2338950.05.4118145
CGTGTAG1999400.05.3301086145
GATCGGA3007250.05.20477145
GTGTACG1094000.05.175498145
AGATCGG3229350.05.071303145
AGAGCGT2673600.05.0218415145
Produced by FastQC (version 0.11.5)