FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002855527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002855527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404174940
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC7110950.1759374294705159Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1540550.072.643069
GAGCGTC1871850.059.849578
ATCGGAA2004150.056.5376781
TCGGAAG2130400.053.136692
AGAGCGT2171250.051.723747
CGGAAGA2373350.047.6089483
AAGAGCG2639250.042.9119346
GAAGAGC5254600.022.0292135
CGCCGTA1243950.017.77168355-59
TCGCCGT1325450.016.65376955-59
GTCGCCG1471000.015.51057455-59
GCGTCGT1466800.015.33676810-14
GCCGTAT1463700.015.24520955-59
CGTCGTG1517600.014.841536510-14
GGAAGAG8215100.014.4909394
TGGTCGC1603800.014.35798650-54
CCGTATC1571600.014.111814555-59
GGTCGCC1656450.013.87798950-54
GTGGTCG1687050.013.75259450-54
CGTATCA1661050.013.48218360-64
Produced by FastQC (version 0.11.5)