FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002855618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002855618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435079093
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG4988030.11464651095059629TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2454750.036.2241631
TCGGAAG2507800.035.5008432
CGGAAGA2647600.033.65293
AGAGCAC5146650.017.702127
AGCACAC5401950.016.9674789
TATGCCG1082550.016.86312745-49
GAGCACA5550600.016.4660538
GAAGAGC5765100.015.9112235
CGTATGC1192250.015.13879845-49
CTCGTAT1204600.015.02053940-44
TCTCGTA1131150.014.21243740-44
ATGCCGT1294100.013.82516645-49
TCGTATG1324350.013.62950540-44
TGCCGTC1287000.013.53181245-49
GCCGTCT1294150.013.39143250-54
TATCTCG1193100.013.24595240-44
ATCTCGT1356550.011.93112740-44
AAGAGCA8227900.011.4401356
CGTCTTC1570600.011.34463950-54
CCGTCTT1583450.011.27913950-54
Produced by FastQC (version 0.11.5)