FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002855668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002855668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452367157
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC9120000.2016061479900938Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2469900.069.165949
GAGCGTC2847200.060.107278
ATCGGAA3288550.055.2621541
TCGGAAG3404900.053.2445072
AGAGCGT3243650.052.8836827
CGGAAGA3709100.048.815113
AAGAGCG3852250.046.949486
TCCGATC617700.031.4668983
AGCGGCG837900.030.9751519
TTCCGAT737350.026.852392
GAAGAGC6912100.026.6348515
CCGATCT748900.025.4214344
ATCGGGA722350.023.2229161
GAGCGGC1149350.022.9095258
CTTCCGA879650.022.6169551
CGCCGTA1386500.021.6689655-59
TCGCCGT1402850.020.04377755-59
GTCGCCG1660800.019.57256555-59
GCCGTAT1634750.018.48745355-59
AGAGCGG1489950.017.9157357
Produced by FastQC (version 0.11.5)