FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002855695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002855695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333387407
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG16974710.5091587037659164TruSeq Adapter, Index 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3133300.098.421111
TCGGAAG3254650.094.492692
CGGAAGA3368400.091.14083
AGAGCAC5378200.057.4336177
AGCACAC5658550.054.5848549
GAGCACA5739900.053.872468
GAAGAGC5843050.052.8896035
AAGAGCA7756650.040.004136
GGAAGAG8581300.036.316894
TATGCCG2529500.024.70042645-49
CTCGTAT2300650.023.73895840-44
CGTATGC2606250.023.68604745-49
TCTCGTA2323500.023.40131640-44
AGCGATA2653800.023.37593330-34
CGATAGA2645150.023.14586335-39
ATGCCGT2701400.022.94191445-49
GCGATAG2667250.022.89556530-34
TCGTATG2371350.022.60024540-44
GCCGTCT2692900.022.59509350-54
TGCCGTC2695800.022.59220545-49
Produced by FastQC (version 0.11.5)