FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002855712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002855712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443450953
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC8925770.20127975686185978Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2424500.066.167559
GAGCGTC2819350.056.9830068
ATCGGAA3246450.052.2995831
AGAGCGT3164050.050.8698967
TCGGAAG3350450.050.5248032
CGGAAGA3632550.046.435113
AAGAGCG3760050.044.721946
GAAGAGC6769100.025.3240175
TCCGATC512250.024.143933
CGCCGTA1357200.020.55201355-59
AGCGGCG739950.020.4427499
CCGATCT614050.019.868674
TCGCCGT1365000.019.43232755-59
TTCCGAT670550.018.9413662
GTCGCCG1610750.018.66287855-59
GCCGTAT1559200.017.93311155-59
TGGTCGC1736900.016.7144650-54
GGAAGAG10829300.016.1752284
CTTCCGA809350.016.0063271
GGTCGCC1878000.016.00112550-54
Produced by FastQC (version 0.11.5)