FastQCFastQC Report
Fri 22 Apr 2022
EGAF00002855818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002855818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372470425
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG10667270.2863924028330571TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2028700.098.311061
TCGGAAG2140000.093.149792
CGGAAGA2275950.087.397313
AGAGCAC4571250.043.8903737
AGCACAC4882700.041.097689
GAGCACA4946450.040.553588
GAAGAGC5047650.039.8603445
AAGAGCA7232300.028.0963696
GGAAGAG7884750.025.8997734
TATGCCG1731400.023.15771345-49
TCGTATC1837150.021.8465535-39
CGTATGC1818700.021.8292845-49
CTCGTAT1589750.021.31330140-44
TCTCGTA1609950.021.0062640-44
GTCACGA1926950.020.97424925-29
ATGCCGT1916650.020.849145-49
TGCCGTC1907700.020.62239845-49
TATCTCG1644400.020.32813640-44
GCCGTCT1923550.020.30214550-54
TCGTATG1680650.019.81462940-44
Produced by FastQC (version 0.11.5)