FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002855848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002855848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403990135
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC4148120.10267874486588639Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1460250.043.4011659
GAGCGTC1779950.035.753018
ATCGGAA2041250.031.7866131
AGAGCGT2137450.029.8780617
TCGGAAG2173200.029.8437292
CGGAAGA2423250.026.6924173
AAGAGCG2611650.024.646526
TCCGATC378250.015.5632743
CGCCGTA872050.015.05048855-59
TCGCCGT940250.013.95727555-59
GTCGCCG1042000.012.62219555-59
GAAGAGC5338150.012.5850885
GCCGTAT1108200.012.07229455-59
CCGATCT504500.011.4964684
TGGTCGC1228300.010.99459550-54
CCGTATC1186850.010.99252855-59
TTCCGAT552250.010.8697242
GGTCGCC1220450.010.76355350-54
CGTATCA1294400.010.30320560-64
GTGGTCG1369950.09.93186350-54
Produced by FastQC (version 0.11.5)