FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002855860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002855860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392430301
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC5658940.1442024223302777Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1530100.059.6206939
GAGCGTC1836250.049.8182688
ATCGGAA1965950.046.6879961
TCGGAAG2111150.043.517412
AGAGCGT2119950.043.2505847
CGGAAGA2331800.039.339733
AAGAGCG2518100.036.360016
GAAGAGC5113650.018.5085835
CGCCGTA1121350.015.96136355-59
TCGCCGT1223650.014.65064655-59
GCCGTAT1326300.013.6424355-59
GTCGCCG1384400.013.40388755-59
CCGTATC1416800.012.71780155-59
GCGTCGT1444100.012.65730910-14
TGGTCGC1523600.012.35715850-54
CGTCGTG1497950.012.23325510-14
CGTATCA1500400.012.0324160-64
GGTCGCC1572800.011.9567850-54
GGAAGAG8375550.011.6490124
GTGGTCG1652550.011.47273550-54
Produced by FastQC (version 0.11.5)