FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002855869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002855869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356423825
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG8681060.24356003698686532TruSeq Adapter, Index 8 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2587400.074.90461
TCGGAAG2695250.071.9214862
CGGAAGA2813900.068.808593
AGAGCAC4981700.039.2214667
AGCACAC5279650.037.0237739
GAGCACA5352850.036.517438
GAAGAGC5484950.035.6785665
AAGAGCA7544950.026.182116
GGAAGAG8332600.023.8516794
CGCGCAT1683300.023.47113835-39
TCGCGCA1747450.022.99950435-39
TCTCGCG1780800.022.85651430-34
TATGCCG1822550.022.48547745-49
GCGCATC1828500.021.73181335-39
CGTATGC1902650.021.29042645-49
CTCGCGC1909150.020.19587530-34
ATGCCGT2009250.020.15076845-49
CTCGTAT1495950.020.04395740-44
GCCGTCT2003950.019.7173150-54
TCTCGTA1504250.019.59309240-44
Produced by FastQC (version 0.11.5)