FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002855891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002855891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410193432
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG19629200.47853520970077357TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3967150.086.540341
TCGGAAG4100600.083.471442
CGGAAGA4214200.081.100673
AGAGCAC6565850.052.3646287
AGCACAC6871750.050.067669
GAGCACA6994150.049.2198948
GAAGAGC7145550.048.1922235
AAGAGCA9476600.036.5398756
GGAAGAG10583400.032.8767784
TATGCCG2870800.024.30017945-49
CTCGTAT2695600.023.27281640-44
CGTATGC2984150.023.1692645-49
TCGTATC3015200.023.05559235-39
TCTCGTA2730550.022.93405740-44
ATGCCGT3080550.022.50303845-49
TATCTCG2802250.022.0637840-44
TGCCGTC3097250.021.91697145-49
GCCGTCT3120700.021.8713950-54
TCGTATG2822700.021.72624640-44
GTCACGA3234200.021.6017725-29
Produced by FastQC (version 0.11.5)