FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002855899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002855899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362707184
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG4258600.11741151506941203TruSeq Adapter, Index 8 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1432400.056.306091
TCGGAAG1535600.052.3963362
CGGAAGA1678100.047.931943
AGAGCAC3849350.021.3884057
AGCACAC4115600.020.0558419
GAGCACA4214050.019.6268428
CGCGCAT804750.019.4904835-39
GAAGAGC4270000.019.3764845
TCGCGCA837250.018.90186935-39
TCTCGCG877400.018.40212630-34
TATGCCG939450.017.1974345-49
GCGCATC945800.016.75701735-39
CTCGCGC991350.016.2035530-34
CGTATGC1016250.015.85641745-49
CTCGTAT928800.015.35456140-44
TCTCGTA949950.014.84177340-44
ATGCCGT1138150.014.21163745-49
TGCCGTC1133350.014.12088345-49
ACTCTCG1182600.013.80745530-34
GCCGTCT1156150.013.77482650-54
Produced by FastQC (version 0.11.5)