FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002855990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002855990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391237439
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC6745100.17240425704759815Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1992100.054.168729
GAGCGTC2328950.046.3457038
AGAGCGT2672650.040.4879537
ATCGGAA2706300.040.1413541
TCGGAAG2813700.038.5415882
CGGAAGA3015750.035.9126173
AAGAGCG3200450.033.8932576
GAAGAGC5716250.019.4431255
CGCCGTA1215650.018.9559555-59
TCGCCGT1313300.017.54538255-59
GCCGTAT1421550.016.34614455-59
GTCGCCG1465250.016.1370155-59
TGGTCGC1593000.015.04303350-54
CGTATCA1604800.014.47901760-64
GTGGTCG1681250.014.35777550-54
CCGTATC1533950.013.85100555-59
GGTCGCC1640700.013.39753250-54
GGAAGAG8690500.013.0920574
GCGTCGT1855700.012.71550710-14
CGTCGTG1892900.012.48246610-14
Produced by FastQC (version 0.11.5)