FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002861376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002861376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5539662
Sequences flagged as poor quality0
Sequence length101
%GC37

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG481870.8698545145895183Illumina Paired End PCR Primer 2 (100% over 31bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT177240.31994731808547167Illumina Paired End PCR Primer 2 (100% over 50bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAG105070.1896686115506686Illumina Paired End PCR Primer 2 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)