FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002861377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002861377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5539662
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT399770.7216505266927838Illumina Single End PCR Primer 1 (100% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG155960.28153342207520965Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCATTCCTGCTGAACCGCTCTTCCGAGATCGGAAGAGCGTCGTGTAGG93560.16889117061654663Illumina Paired End Adapter 2 (96% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71450.12897898824874154No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)