FastQCFastQC Report
Fri 6 Jan 2023
EGAF00002861777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002861777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44195661
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATTCGCATCTCGTA6785971.5354380603109432Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGC3315450.7501754527441054Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGCA489260.11070317513748691Illumina Paired End Adapter 2 (96% over 28bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGA474430.10734764211355499Illumina Paired End PCR Primer 2 (100% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)