FastQCFastQC Report
Wed 22 Feb 2023
EGAF00002861873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002861873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48233567
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGACCTATCTCGTA1325110.27472776375838015Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGA606250.12569047609520564Illumina Paired End PCR Primer 2 (100% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)